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KMID : 0895820140240020031
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Journal of Oriental Rehabilitation Medicine
2014 Volume.24 No. 2 p.31 ~ p.40
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Effects of Trachelospermum caulis Extract on Sodium Nitroprusside (SNP)-induced Inflammatory Responses in Rabbit HIG-82 Synovial Membrane Cells
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Park Jung-Sik
Lim Hyung-Ho
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Abstract
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Objectives : Trachelospermi caulis, known as Nak-Suk-Deung in Korea, is the dried leafy stem of Trachelospermum asiaticum var. intermedium Nakai, and climbing stems and branches of Trachelospermum sdisyivum var, intermedium nakai or Apocyanaceae. Trachelospermi caulis has antipyretic and analgesic activity. It has traditionally been used as a folk remedy in Korea for the treatment of various infla mMatory diseases, including rheumatoid arthritis. The purpose of this study was to evaluate the Effects of Trachelosper-mum caulis extract on SNP-induced infla mMatory responses in rabbit HIG-82 synovial membrane cells.
Methods : Anti-infla mMatory effects of the extract of Trachelospermum caulis were inves-tigated using rabbit HIG-82 synovial membrane cells. For this study, 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, western blot analysis, PGE2 i mMuno-assay, and NO detection were conducted.
Results : The aqueous extract of Trachelospermum caulis exerted cytotoxicity and sup-pressed PGE2 synthesis and NO production in rabbit HIG-82 synovial membrane cells. The aqueous extract of Trachelospermum caulis also inhibited the SNP-induced expressions of COX-2, iNOS, and TNF-α in rabbit HIG-82 synovial membrane cells.
Conclusions : These results showed that the extract of Trachelospermum caulis exerts the anti-infla mMatory effect by suppressing COX-2, iNOS, and TNF-α expressions in the synovial membrane cells.
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KEYWORD
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Trachelospermum caulis, infla mMation, COX-2, iNOS, TNF-α, PGE2, NO
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